Scientists alter sexual orientation in worms

University of Utah biologists genetically manipulated nematode worms so the animals were attracted to worms of the same sex - part of a study that shows sexual orientation is wired in the creatures' brains. 'They look like girls, but act and think like boys,' says Jamie White, a postdoctoral researcher and first author of the new study. 'The [same-sex attraction] behaviour is part of the nervous system.' The study was published online today in Current Biology, and will run in the journal's 6 November print edition.

'The conclusion is that sexual attraction is wired into brain circuits common to both sexes of worms, and is not caused solely by extra nerve cells added to the male or female brain,' says laboratory leader and biology Professor Erik Jorgensen, scientific director of the Brain Institute at the University of Utah and an investigator with the Howard Hughes Medical Institute.

'The reason males and females behave differently is that the same nerve cells have been rewired to alter sexual preference,' he adds. 'Our conclusions are narrow in that they are about worms and how attraction behaviours are derived from the same brain circuit. But an evolutionary biologist will consider this to be a potentially common mechanism for sexual attraction.'

'We cannot say what this means for human sexual orientation, but it raises the possibility that sexual preference is wired in the brain,' Jorgensen says. 'Humans are subject to evolutionary forces just like worms. It seems possible that if sexual orientation is genetically wired in worms, it would be in people too. Humans have free will, so the picture is more complicated in people.'

White and Jorgensen conducted the study with technician Jeff Gritton and three University of Utah biology undergraduates: Thomas Nicholas, Long Truong and Eliott Davidson. The study was funded primarily by the National Science Foundation.

Nematodes, or C. elegans, are millimetre-long (one twenty-fifth of an inch) worms that live in soil and eat bacteria. Because the same genes are found in many animals, nematodes, mice, zebrafish and fruit flies often are used as 'models' for humans in research.

Nematode worms lack eyes, so attraction is based only on the sense of smell. There are no true females and only one in 500 nematodes is male. Most are hermaphrodites, with both male and female organs. Jorgensen and White loosely refer to hermaphrodites as females because they produce offspring.

'A hermaphrodite makes both eggs and sperm,' Jorgensen says. 'She doesn't need to mate [with a male] to have progeny,' but can fertilise her own eggs. 'Most of the time, the hermaphrodites do not mate. But if they mate, instead of having 200 progeny, they can have 1,200 progeny.'

Nematodes are few and far between in soil. So natural selection favoured hermaphroditic worms because when they found an abundant food source, they were able to feast and make babies even if no males were nearby, Jorgensen says.

Male nematodes must find hermaphrodites if they are to reproduce, and they find them by smelling their sex-attractant odours or pheromones.

Jorgensen says the study looked at three possibilities, namely, whether male attraction to hermaphrodites results from:

- Four 'accessory' or extra smell-related nerve cells named CEM neurones that are found only in male brains. The worms gain these neurones during their version of puberty, their fourth larval stage.

- Four 'core' or basic smell-related nerve cells - two named AWC and two named AWA - found in both males and hermaphrodites.

- Both the accessory and core neurones.

The answer was that male attraction involves the combination of both accessory and core nerve cells. The involvement of the core neurones was a surprise.

'We thought the extra CEM neurones provided sexual preference' because fourth-stage males are not attracted to hermaphrodites but adult males are, Jorgensen says. 'We found instead that the brain - which is the same in young males and hermaphrodites - is rewired during the worm equivalent of puberty - the fourth larval stage - to make the males attracted to hermaphrodites.'

'What we show is that the shared nervous system [common between male and hermaphrodite] is broadly sexualised,' and sexual attraction can be changed by essentially flipping a genetic switch in that common brain, he adds.

The study involved these key experiments:

- The researchers used laser microsurgery to kill the male-only CEM neurones in young larval males. The resulting adult males still were attracted to hermaphrodites.

That suggests the core neurones - brain cells common to both the male and hermaphrodite brain - are sexualised, and that the extra CEM nerve cells found only in males are not necessary for sexual attraction even though they normally play a role in it.

- The biologists zapped eight different kinds of nerve cells involved in the sense of smell and taste: the four kinds of CEM neurones found only in males and four kinds of core neurones (AWA and AWC) also found in hermaphrodites. If any of the eight types of neurones was damaged in adult males, attraction was impaired. But when the nerve cells were zapped before puberty in fourth-stage larvae, they grew into adults with normal attraction to hermaphrodites. That shows 'the nervous system can compensate for lost neurones as it goes through puberty,' Jorgensen says.

'Normally there are eight sensory neurones in nematodes,' says White. 'You can take away seven of the eight prior to sexual maturation, and as long as there is one left, he can still be sexually attracted... Why would an organism that has only 383 nerve cells use eight of them for sexual attraction? It must be that the behaviour is very important. There is redundancy. The system is flexible.'

- Next, 'we took the hermaphrodite brain and we activated the genes that determine maleness,' but only in the brain and not in the rest of the worm, Jorgensen says. Hermaphrodites with masculine brains 'were attracted to other hermaphrodites.'

The results show sexual orientation is wired into the brain in both sexes of worm.

To masculinise the brains of hermaphroditic worms, the researchers activated a gene named fem-3, but only in the nervous system. The fem-3 gene makes the body develop male structures such as a tail, which male worms use for copulation. With the gene active only in the brain, the hermaphrodites still had the same bodies and genitalia, but their brains were male, so they were attracted to other hermaphrodites.

To demonstrate the hermaphrodites produce sexual attractants or pheromones, the researchers washed hermaphrodites, and put some of the wash water on agar, a jelly-like growth medium, in a culture dish. When worms were placed on the dish, males moved toward the hermaphrodite wash water while hermaphrodites moved away.

When the scientists genetically altered hermaphrodites' brains to change their sexual orientation, they crawled toward the pheromones of other hermaphrodites.

'People debate whether the brain is influenced by sexual hormones from the gonads or whether the behaviour is derived from the brain alone,' Jorgensen says. 'In this case, it's clear the brain is sexualised... The surprise was that sensory neurones found in the hermaphrodite brain are involved in sexual attraction in males.'